How to choose the light source of the fluorescence microscope?

When choosing the source of the fluorescence microscope (Fluorescence Microscope) , we should pay attention to the size of the arc, which is the size of the light-emitting area of ​​the light source. For example, a 50W mercury lamp has an arc size of 110X0.13MM and a 200W arc size of 212X0. 15MM, and the 100W arc size is 0.125×0.125m, can it be fully filled with the system to make full use of light energy, for an excellent fluorescence microscope ( Fluorescence Microscope ) , and a variety of magnification objective lens "light energy" Full and effective use is an indicator worthy of attention. In fluorescence microscopy design, the choice of source is critical in the overall design, which determines the scope and cost of the instrument and also reflects the instrument's grade. Microscopes above the laboratory should be based on mercury lamps , while simple (special) microscopes often use halogen lamps as the main source.

Secondly, the question we have to consider is its power. Is it a high-powered light bulb or a low-wattage light bulb? In principle, a low-wattage light source should be used as much as possible, which is undoubtedly beneficial to the structural design and cost. of. However, the wattage that is too low cannot be excited by excitation or the fluorescence emitted is unstable, so it cannot be used. However, whether fluorescence can be excited is related to the utilization of light energy in the entire imaging system. How to fully and effectively use light energy, the wattage can be reduced, but if we simply pursue high-power light source as fluorescence detection, it is not desirable. Because it causes fluorescence decay and annihilation. Currently available epi-fluorescence microscopes are only equipped with 100W mercury or xenon and halogen lamps .