Objective: To establish a standard operating procedure for clean room disinfection of sterile rooms, to provide standards for the cleaning and disinfection operation of the sterile room of the quality inspection center, to prevent pollution, and to ensure the accuracy and authenticity of drug quality control and inspection.
Scope: Suitable for cleaning and disinfecting the laboratory's sterile room. The staff of the sterile room of the quality inspection center is responsible for the implementation of this regulation, and the quality assurance department is responsible for supervision.
Sterilization and sterilization operation essentials
1. Disinfect the sterile room space and turn on the UV lamp for 30-60 minutes.
2. The relevant equipment for inspection must be sterilized separately before being moved into the sterile room.
3. The operator must clean and disinfect the hands and wear sterile work clothes, caps and shoes to enter the sterile room.
4. After entering the sterile room, disinfect the hands again, and then carry out the inspection operation.
Operational process precautions
1, the action should be light, not too fast, so as not to stir the air to increase pollution; glassware should also be taken lightly to avoid damage to the environment.
2. The operation should be carried out in the near flame zone.
3, inoculation rings, inoculation needles and other metal equipment need to be burned before and after use, first through the internal flame after burning, so that the residue is dried and then burned and sterilized.
4. When using the straw, do not directly suck and blow the straw with the mouth, but must use the ear wash.
5. It is not good to open the lid when observing the plate. If you want to take the colony inspection, you must operate close to the flame zone. The flat lid cannot be opened wide, but the upper and lower lids should be properly slit.
6. When staining suspicious pathogenic bacteria, use clips to hold the slides. Do not take the slides directly with your hands to avoid contamination. Used slides should be placed in disinfectant for disinfection, and then washing.
7. At the end of the work, clean up the samples and equipment on the workbench, and wipe the workbench with disinfectant after the Zui.
Sterilization method
Disinfection of the sterile room can be done in the following ways:
1, mixed with formaldehyde and potassium permanganate fumigation: generally 40% formaldehyde per square meter 10 ml, potassium permanganate 8 ml, fumigation. When using, first close the doors and windows, the amount of formaldehyde solution into the container, and then pour a good amount of potassium permanganate, the staff will leave the inoculation room, close the door, fumigation for 20-30 minutes.
2, 0.1% water disinfection: use 0.1% liter of water soaked gauze or sponge for finger rubbing, or spray spray sterilization, so that the upper and lower sides of the box are stained with water, hands can also be disinfected with water, and the sleeves are rolled up . 20 to 30 minutes after the spraying, the bacteria and mist in the box fell to the bottom of the box and were killed, and the internal air became very clean.
3, UV irradiation sterilization: in the aseptic box is equipped with a 200 volt, 3O watt UV lamp; each time for 20 to 30 minutes, you can achieve the purpose of space sterilization. After the end of the irradiation, cover the black cloth for half an hour to enhance the sterilization effect.
4, carbolic acid spray: before each inoculation, spray with 5% carbolic acid solution, can promote the sedimentation of particles and bacteria in the air, prevent desktop dust from flying, and have a bactericidal effect.
5, lime rubbing: often with drugs fumigation, easy to cause acidic environment, especially with formaldehyde and potassium permanganate fumigation for a long time, pollution is often more and more serious, preventive methods can be used interchangeably, after a period of time (about five Week) scrub with lime. Practice has proved that this works well.
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