First, the main raw materials
1. Cordyceps strains: They can be used directly by professional scientific research units or manufacturers for the purchase of Cordyceps militaris and their original species, and can also be used for production after testing.
2. Live silkworms: Live silkworms that are robust and have a uniform length and thickness should be selected from 3 to 4 years of age. It can be purchased from silkworm farms and silkworm farmers, and it can also be purchased for silkworm breeding.
3. Cordyceps special antifouling agent: It is an antifouling additive for seed cultivation, mainly used in the medium to enhance antifouling power.
4. Live silkworm special disinfectant: for the disinfection of live silkworm body, to prevent bacterial contamination caused by bacteria.
Second, the production of Cordyceps species
1. The preparation of culture medium: rice 90%, silkworm meal powder 4%, peptone 2%, glucose 2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B110 mg, Cordyceps special antifouling agent 0.3 % to 05%, pH value 7, material to water ratio of 1:1.3 to 1.4. The silkworm cocoon powder is powder dried after eating silk cocoon or silk cocoon, dried or dried and ground.
Weigh 30 to 50 grams of rice according to the proportion of the above medium formulation, and put it into a bag or can of polypropylene folding angle plastic (10665, 230.00, 2.20%) with a thickness of 0.5 mm or more and 15 cm 25 cm. One to two grams of silkworm cocoon powder is placed in a plastic bag (bottle) containing rice, and then a nutrient solution is prepared. That is, glucose, potassium dihydrogen phosphate, magnesium sulfate, and vitamin B1 are added to the water according to the ratio of water to water. Stir and dissolve completely to make the pH 7. Measure the nutrient solution into a plastic bag (bottle) containing rice and silkworm cocoons, seal the plastic bag (bottle), and soak for 2 to 3 hours, and transfer the common aluminum (iron) pot with appropriate amount of water. Ordinary pressure sterilizing pot or autoclave sterilization, when the temperature reaches 100 °C for 1 to 2 hours, steamed cooked rice blocks stop heating, wait until after cooling can access bacteria.
2. Inoculation, culture: Inoculation items, inoculum strains, culture medium, etc., which are likely to cause contamination, are placed in the inoculation room and sterilized by a conventional method or using an aerosol disinfectant for 30 minutes. When inoculated, Cordyceps militaris and the original seedlings are wiped and sterilized with 75% alcohol, etc., and then the disinfected inoculum hooks or scorpions are used to dig the finger and size blocks, and the strains are inserted into 2 to 3 pieces. Ample, can increase the amount of bacteria. After inoculation, it should be garnetted as soon as possible, and it should be moved into the culture room for shade culture. The optimum temperature was about 18°C. The mycelium began to germinate about 1 day after culture. The feed was started in about 2 days. The growth of mycelia should be checked in 7 to 10 days. The temperature should be raised when the temperature is low, and the temperature should be lowered or ventilated when the temperature is higher than 25°C. The mycelium can be filled with plastic bags (bottles) for about 20 to 30 days under proper temperature conditions. The bacteria can be used after being grown, or stored in a room at a lower temperature.
Third, technical methods
1. Silkworm body disinfection: first, the silkworm body special disinfectant is dissolved in clean cold water according to the amount of 2%, and it is repeatedly agitated. After it is completely dissolved, the living silkworm is put into the disinfecting liquid, removed after disinfection, and the water is drained off. , with the strains into the cultivation room, after inoculation can be inoculated after cultivation.
2. Cordyceps cultivating method: When cultivated, it should be sterilized according to the requirements and methods of inoculation, and use the sterilized tools to dig bacteria species and spread it on the disinfected plastic film, cover it, and tightly press it around. This method can be used plane method can also be used shelf-bed planting method, the temperature is maintained at 20 ~ 22 °C, cultured for about 10 days, Cordyceps mycelium can be completely infected with the body, when the body began to harden, the insects can be Dispose on the plastic film that has been disinfected or in plastic bags (bottles) for moisturizing culture. According to the growth conditions, give suitable temperature, humidity, light, ventilation, temperature difference, etc. according to the conventional method of Cordyceps cultivation, and let them be produced. Subbase. It is also possible to mix and sterilize the sterilized silkworm body with the bacteria in the plastic bag (bottle) until it is infected by the mycelium. Then pick it out into a sterilized plastic bag (bottle). When the sub-seat grows about 7 to 8 centimeters, the sub-seats and insects can be prevented from being separated when harvested. The harvested cordyceps should be air-dried or dried and stored in a closed plastic bag.
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