The Thermo Scientific GC/MS is an instrument that combines a gas chromatograph with a mass spectrometer. Mass spectrometry can perform effective qualitative analysis, but the analysis of complex organic compounds is powerless; chromatography is an effective separation method for organic compounds, especially suitable for quantitative analysis of organic compounds, but qualitative analysis is more difficult. Therefore, the effective combination of the two will provide chemists and biochemists with a highly efficient qualitative and quantitative analysis tool for complex organic compounds. A technique that combines two or more methods like this is called a joint technique.
Application sharing of Thermo Fisher's GC/MS
The Thermo Scientific GC/MS is widely used in the separation and identification of complex components. It has high resolution of GC and high sensitivity of mass spectrometry. It is an effective tool for qualitative and quantitative determination of drugs and metabolites in biological samples.
The basic components of the Thermo Scientific GC/MS are: three-part, they are placed in the vacuum manifold.
Interface: The sample from the GC enters the mass spectrometer through the interface, which is the key to the GC/MS system.
The interface function of the Thermo Scientific GC/MS
1 Pressure Matching - The mass spectrometer ion source has a vacuum of 10-3 Pa, and the GC column interface is designed to match the pressure between the two.
2 Concentration of components - There is a large amount of carrier gas in the gas flowing out of the GC column. The interface is used to remove the carrier gas and concentrate the analyte into the ion source.
Common interface technology of Thermo Fisher's GC/MS
1 molecular separator connection (mainly used for packed columns)
Diffusion type - The rate of diffusion is inversely proportional to the square of the molecular weight of the substance and is proportional to its partial pressure. When the chromatographic effluent passes through the separator, the carrier gas of the small molecule is easily diffused out of the micropores and is removed by the vacuum pump, and the measured object has a large molecular weight and is not easily diffused to be concentrated.
2 direct connection method (mainly used for capillary columns)
A column of stainless steel capillary with a length of about 50 cm and an inner diameter of 0.5 mm is connected between the column and the ion source, and the chromatographic effluent passes through the capillary to the ion source. This interface technology has high utilization rate.
3 open shunt connection
The interface is to vent a portion of the chromatographic effluent and allow another portion to enter the mass spectrometer and carry the excess effluent away by continuously flowing into the cleaning helium. This method has low sample utilization.
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Chlamydia pneumoniae is an important cause of acute respiratory infections, but may also be associated with chronic diseases (24). Because chlamydia pneumoniae was different from other types of chlamydia, a new name was proposed for the bacterium, chlamydia pneumoniae. Although the overall incidence is unknown, there are an estimated 2 to 5 million cases of pneumonia and 500,000 pneumonia-related hospitalizations in the United States each year, according to CDC estimates. While all age groups are at risk, it is most common in school-age children. In addition, according to some researchers, mycoplasma pneumoniae infection may be associated with atherosclerotic vascular disease, as well as Alzheimer's disease, asthma, and reactive arthritis.
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