The mother breed is mostly cultured in semi-synthetic medium. There should be a plan for the production of mother plants, ie the number of production species to be used in a certain period of time, in order to determine the number of mother plants to be produced. The first generation of mother-species as much as possible out of the inclined pipe, reducing the generation of the turn of the tube, to avoid the mycelium decreased viability, low mushroom rate and other phenomena. The inclined pipe can be stored for 3 months in a refrigerator at 4 degrees Celsius, but it is best used within a month. The beveled surface with paraffin oil can be stored longer at 4 degrees Celsius. Before expanding production, the mother plant must be strictly inspected for contamination of pests and diseases, whether the label is complete, and whether it has undergone comprehensive evaluation of mushrooming tests before it can be expanded. For the first time, the mother-to-child transfer tube can transfer 60-80 tubes for the second time. Part of the second mother breed is used for transfer of the original species and is partially preserved. In the same way, the average female parent is passed on from 3-4 times.
Inclined parent species transfer method: The transfer process should be performed in a sterile or sterile room. Staff, work environment, and all equipment must be strictly disinfected or sterilized. Before the inoculation, the bevel tube was wiped with 75% alcohol. Next to the alcohol lamp, the tampon was pulled out between the fingers of the hand holding the inoculation spatula. The flame was gently burned before the tube was transferred. The sterilized and slightly cooler inoculum was used. The spatula is first longitudinally cut into a 2 mm wide strip on the mother species slant, and then shoveled off with an inoculation scoop about 2-3 mm deep along the horizontal direction of the slant, and then cut horizontally into 2 mm wide and 4 mm in length with an inoculation spatula. Millimeters of small pieces. Use an inoculum spatula to remove the approximately 1 cm section of the oblique front section. The remaining species of bacteria are inserted into the blank slant culture medium one by one. The bacterial species is placed in the middle of the blank incline. Burn the nozzle and stuff it with a tampon. The above operation is performed under the condition of no bacteria, which is called aseptic operation. Leave the alcohol lamp and write a label. The newly added strains are immediately cultivated at a suitable temperature. When the mycelium of the original mycelia germinates a new mycelium, the original temperature can be lowered by 2-3 degrees Celsius, so that the mycelium grows slowly and will be stronger and more vital. To the mycelium covered with slant is the strain mature.
Inclined parent species transfer method: The transfer process should be performed in a sterile or sterile room. Staff, work environment, and all equipment must be strictly disinfected or sterilized. Before the inoculation, the bevel tube was wiped with 75% alcohol. Next to the alcohol lamp, the tampon was pulled out between the fingers of the hand holding the inoculation spatula. The flame was gently burned before the tube was transferred. The sterilized and slightly cooler inoculum was used. The spatula is first longitudinally cut into a 2 mm wide strip on the mother species slant, and then shoveled off with an inoculation scoop about 2-3 mm deep along the horizontal direction of the slant, and then cut horizontally into 2 mm wide and 4 mm in length with an inoculation spatula. Millimeters of small pieces. Use an inoculum spatula to remove the approximately 1 cm section of the oblique front section. The remaining species of bacteria are inserted into the blank slant culture medium one by one. The bacterial species is placed in the middle of the blank incline. Burn the nozzle and stuff it with a tampon. The above operation is performed under the condition of no bacteria, which is called aseptic operation. Leave the alcohol lamp and write a label. The newly added strains are immediately cultivated at a suitable temperature. When the mycelium of the original mycelia germinates a new mycelium, the original temperature can be lowered by 2-3 degrees Celsius, so that the mycelium grows slowly and will be stronger and more vital. To the mycelium covered with slant is the strain mature.
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